Journal:

Article Title: Guanosine supplementation reduces apoptosis and protects renal function in the setting of ischemic injury

doi:

Figure Lengend Snippet: Effects of guanosine and enhanced GTP recovery on renal cell apoptosis at 24 hours. Mice were treated as detailed in legend of Figure ​Figure2.2. Kidney sections were obtained at 24 hours and costained with TUNEL and DAPI. (a) A negative control (NC; TdT omitted) and (b) a DNase-treated positive control (PC) for TUNEL. All TUNEL-positive nuclei had bright green fluorescence. (c) A representative field showing the medulla from NS/I group. Extensive TUNEL-positive staining is seen and is localized primarily to the tubular epithelium (dark green autofluorescence). (d) A representative field from the medulla of the G/I group showing lack of TUNEL-positive staining. (e) A magnification of TUNEL-positive nuclei from NS/I mice, with only the DAPI channel turned on. ×60. Nuclei are condensed, dysmorphic, and show heavy staining. Inset shows a single nucleus. ×120. Typical apoptotic fragmentation of chromatin into four small bodies is seen. (f) A magnification of TUNEL-negative nuclei from G/I mice. ×60. They show lack of condensation and faint DAPI staining.

Article Snippet: Finally, separate sections were stained with TUNEL reagent (Promega Corp., Madison, Wisconsin, USA) and DAPI for in situ apoptosis detection.

Techniques: TUNEL Assay, Negative Control, Positive Control, Fluorescence, Staining

Journal:

Article Title: Guanosine supplementation reduces apoptosis and protects renal function in the setting of ischemic injury

doi:

Figure Lengend Snippet: Quantitative evaluation of TUNEL-positive nuclei in cortex and medulla. Values are means ± SE and represent number of TUNEL-positive nuclei expressed as percentage of total number of nuclei. Mice were treated as detailed in the legend of Figure ​Figure2.2. Kidney sections were obtained at 24 hours and processed for TUNEL and DAPI staining as detailed in Methods. Number of fields counted: 20 for G/I, 35 for NS/I, 8 for G/sham, and 8 for NS/sham. (*P < 0.01 when medullary TUNEL-positive nuclei in NS/I were compared to all other groups).

Article Snippet: Finally, separate sections were stained with TUNEL reagent (Promega Corp., Madison, Wisconsin, USA) and DAPI for in situ apoptosis detection.

Techniques: TUNEL Assay, Staining

Journal:

Article Title: Guanosine supplementation reduces apoptosis and protects renal function in the setting of ischemic injury

doi:

Figure Lengend Snippet: Distribution of TUNEL-positive nuclei among various nephronal segments. (a) A 5-μm thick section from NS/I mice stained with TUNEL and DAPI. The TUNEL positive nuclei were pseudocolored white instead of green to allow clear distinction from the green FITC-phalloidin. (b) A 5-μm section immediately contiguous to the one shown in a is stained with the actin marker FITC-phalloidin (bright green) to identify proximal tubules (P). Distal tubules (D) show minimal or no staining. The section was costained with anti-THP Ab and a Cy5-conjugated secondary Ab (red). This is predominantly a marker for Henle’s loop segments (LH). (c) An overlay of a and b allows the localization of TUNEL-positive nuclei to various tubular segments. (d) A quantitative estimate of the distribution of TUNEL-positive nuclei normalized to total number of DAPI-positive nuclei for each particular tubular segment or lumen. Other refers to interstitial or vascular TUNEL-positive nuclei.

Article Snippet: Finally, separate sections were stained with TUNEL reagent (Promega Corp., Madison, Wisconsin, USA) and DAPI for in situ apoptosis detection.

Techniques: TUNEL Assay, Staining, Marker